Generator

Part:BBa_K821012:Design

Designed by: Jinlai Zhang, Dongchang Qin   Group: iGEM12_Tianjin   (2012-09-18)


PBad(promoter)+orthogonal 16S RNA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2207
    Illegal BamHI site found at 1260
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1095
    Illegal AgeI site found at 2221
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1077


Design Notes

Induce the expression of the orthogonal 16S RNA


Source

From genomic sequence rrnB

References